Alginate Particles with Ovalbumin (OVA) Peptide Can Serve as a Carrier and Adjuvant for Immune Therapy in B16-OVA Cancer Model
Longbao Zhu, Fei Ge, Liangjun Yang, Wanzhen Li, Shenghua Wei, Yugui Tao, Guocheng Du
(School of Biochemical Engineering, Anhui Polytechnic University, Wuhu, Anhui, China (mainland))
Med Sci Monit Basic Res 2017; 23:166-172
Alginate is a natural polysaccharide obtained from brown algae and has been shown to have numerous applications in biomedical science, such as wound healing, delivery of bioactive agents, and cell transplantation. Ovalbumin (OVA) peptide 323-339 has been reported to be involved in immune response.
MATERIAL AND METHODS: This work investigated the use of alginate particles as a carrier and adjuvant for the immune therapy of cancer. Alginate particles loaded with OVA peptide were produced via emulsion. A tumor model was established in C57BL/6J mice via subcutaneous injection of 3×105 B16-OVA tumor cells. The effect of alginate/OVA peptide on cell viability was analyzed by use of the CCK-8 assay kit. Activation of macrophages was examined by checking cell surface makers CD40 and CD86 by FACs.
RESULTS: Alginate/OVA peptide inhibited tumor progression more effectively than using the peptide alone. The viability and uptake study illustrated that this particle is safe and non-toxic. The activation study demonstrated that alginate particles can promote the activation of surface markers on macrophages. ELISA assay showed that the particles with peptide can promote the secretion of inflammatory and effector cytokines from macrophages.
CONCLUSIONS: This study demonstrated that alginate has dual functions in immune therapy of cancer, serving both as a carrier and an adjuvant.
Keywords: Alginates, Cytokines, Macrophage Activation, Ovarian Neoplasms