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Elevated expression of C3G protein in the peri-infarct myocardium of rats

Liping Wang, Gang Li, Zhihua Wang, Xiaolan Liu, Wenju Zhao

Med Sci Monit Basic Res 2013; 19:1-5

DOI: 10.12659/MSMBR.883709

Published: 2012-12-31


Background:    The integrin β1 subunit and its downstream molecules such as integrin-linked kinase (ILK) and focal adhesion kinase (FAK) are indispensable to the inhibition of postinfarction cardiac remodeling, ischemic cardiomyopathy, and heart failure. As a component of the integrin pathway, C3G (Crk SH3-domain-binding guanine nucleotide exchange factor) protein may also participate in postinfarction cardiac remodeling, ischemic cardiomyopathy, and heart failure.
    Material/Methods:    Experimental myocardial infarction (MI) and sham-operation (sham) models were set up in Sprague-Dawley rats. C3G protein expression in the myocardium in the sham group and in the non-infarcted myocardium of the peri-infarct zones in the MI group was examined by Western blot.
    Results:    The C3G protein expression in the myocardium was 0.22±0.06, n=8 in the post-sham 24-hour group; 0.29±0.10, n=8 in the post-MI 24-hour group; 0.22±0.07, n=8 in the post-sham 12-week group; and 0.56±0.14, n=8 in the post-MI 12-week group. The C3G protein expression in the myocardium in the post-MI 12-week group was significantly elevated compared to that in the post-sham 12-week group (p=0.0002), in the post-sham 24-hour group (p=0.0002), and in the post-MI 24-hour group (p=0.0006).
    Conclusions:    C3G protein expression exhibits in the myocardium of rats. Furthermore, C3G protein expression is significantly elevated in the non-infarcted myocardium of the peri-infarct zones. The elevated C3G protein expression could participate in postinfarction cardiac remodeling, ischemic cardiomyopathy, and heart failure.

Keywords: Myocardial Infarction - physiopathology, Male, Myocardium - pathology, Immunohistochemistry, Guanine Nucleotide-Releasing Factor 2 - metabolism, Collagen - metabolism, Animals, Rats, Rats, Sprague-Dawley



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